rabbit antibodies against phospho  (Cell Signaling Technology Inc)

Journal: British journal of pharmacology

Article Title: Difluorinated thromboxane A 2 reveals crosstalk between platelet activatory and inhibitory pathways by targeting both the TP and IP receptors.

doi: 10.1111/bph.16435

Figure Lengend Snippet: FIGURE 8 F2-TXA2 induces protein kinase C (PKC) substrate phosphorylation compared with vasodilator-stimulated phosphoprotein phosphorylation (P-VASP). Washed platelets (4 108 platelets per millilitre) were prepared from human volunteers for Western blotting. Platelets were incubated with 10 μM F2-TXA2 or 10 μM U46619 for various time points prior to lysis (a), data normalised to U46619 response at 30 s, mean ± SEM (n = 5) (b). To determine protein kinase A activation, P-VASP Ser239 was used as a readout. Briefly, platelets were incubated with varying concentrations of F2-TXA2 or 3 μM ralinepag or 3 μM iloprost for 5 min prior to lysis (c). To determine PKC activation, P-pleckstrin was used as a downstream readout. Briefly, platelets were incubated with varying concentrations of F2-TXA2 or 10 μM U46619 for 30 s prior to lysis (d). Concentration-dependent activation of P-VASP (% maximal response iloprost) or PKC (% maximal response U46619) was compared, mean ± SEM, n = 5, log (agonist) versus response–variable slope (four parameters) was fitted using GraphPad Prism 9 (EC50 P = 0.0199, unpaired t test, Emax P = 0.1683 unpaired t test, n = 5) (e).

Article Snippet: 2.1.3 | Antibodies Western blotting: anti-talin goat polyclonal antibody (Santa Cruz Bio- technology [Dallas TX, USA] Cat# sc-7534, RRID:AB_661610) or anti- mouse talin-1 antibody (Thermo Fisher Scientific Cat# MA5-28133, RRID:AB_2745116) to probe for talin, anti-PKC substrate rabbit poly- clonal antibody (Cell Signaling Technology [Danvers TX, USA] Cat# 2261, RRID:AB_330310) to probe for P-pleckstrin PKC substrate phosphorylation, anti-phospho vasodilator-stimulated phosphoprotein (P-VASP) Ser239 rabbit polyclonal antibody (Cell Signaling Technology Cat# 3114, RRID:AB_2213396) to probe for protein kinase A (PKA) downstream target phosphorylation.

Techniques: Phospho-proteomics, Western Blot, Incubation, Lysis, Activation Assay, Concentration Assay

Journal: iScience

Article Title: De novo TLK1 and MDM1 mutations in a patient with a neurodevelopmental disorder and immunodeficiency

doi: 10.1016/j.isci.2024.109984

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-phospho-(Ser) PKC Substrate, clone P-S3-101 , Cell Signaling Technology , Cat#6967; RRID: AB_10949977.

Techniques: Virus, Bacteria, Recombinant, Protease Inhibitor, Flow Cytometry, Isolation, DNA Library Preparation, Single Cell Gel Electrophoresis, Plasmid Preparation, Gel Extraction, Ligation, Mutagenesis, Sequencing, Amplification, Software